Isabel Campos Pinto

Dedicated and highly motivated scientist with over 5 years of experience in antibody discovery and engineering, cloning, protein expression and purification, and immunoassay development. Graduated with a degree in Biochemistry from the University of Lisbon (Portugal) in 2012, followed by a MSc degree in Biotechnology from Instituto Superior Técnico (Portugal) in 2014. Obtained a PhD from Aston University (UK) in 2020.

• Towards detection of structurally-diverse glycated epitopes in native proteins: Single-chain antibody directed to non-A1c epitope in human haemoglobin, Gaetani ML, Pinto IC, Li M, O'Connor P, Giorgi-Coll S, Tyreman M, Rumary KL, Schouten JA, Davis P, Dixon AM, Mol Immunol, 02/01/24, 10.1016/j.molimm.2023.12.008, 38181455
• High-throughput proteomics and in vitro functional characterization of the 26 medically most important elapids and vipers from sub-Saharan Africa, Giang Thi Tuyet Nguyen, Carol O'Brien, Yessica Wouters, Lorenzo Seneci, Alex Gallissà-Calzado, Isabel Campos-Pinto, Shirin Ahmadi, Andreas H Laustsen, Anne Ljungars, GigaScience, 11, 2022, https://doi.org/10.1093/gigascience/giac121
• Epitope mapping and characterization of 4-hydroxy-2-nonenal modified-human serum albumin using two different polyclonal antibodies, I. Campos-Pinto, L. Méndez, J. Schouten, J. Wilkins, M. Fedorova, A. Pitt, P. Davis, C. Spickett, Free Radical Biology and Medicine, 2019, https://doi.org/10.1016/j.freeradbiomed.2019.05.008
• LYTAG-driven purification strategies for monoclonal antibodies using quaternary amine ligands as affinity matrices, Campos-Pinto, I., Capela, E. V., Silva-Santos, A. R., Rodríguez, M. A., Gavara, P. R., Fernandez-Lahore, M., Aires-Barros, M. R. and Azevedo, A. M., J. Chem. Technol. Biotechnol, 2017
• Integration of cell harvest with affinity enhanced purification of monoclonal antibodies using aqueous two-phase systems with a dual tag ligand, Campos-Pinto, I., Espitia-Saloma E., Rosa, SA., Rito-Palomares, M., Aguilar, O., Arévalo-Rodríguez, M., Aires-Barros, MR. and Azevedo, AM., Separation and Purification Technology, 2016, 173, 129-134, https://doi.org/10.1016/j.seppur.2016.09.017

• Immunodetection of lipoxidation: epitope mapping of HNE-modified human serum albumin using two different ant-HNE pAbs, MASSTRPLAN Final Conference: Advances in the Study of Lipid and Protein Oxidation: From Methods to Targets, Ghent, Belgium, 03/01/19, Awarded best poster presentation
• Immunodetection of lipoxidation: epitope mapping of HNE-modified human serum albumin using two different ant-HNE pAbs, SFRR –19th International Biennial Meeting, Lisbon, Portugal, 06/01/18
• Detecting lipoxidation: diagnostic tools for translation and commercial application, International HNE Meeting club, Graz, Austria, 09/01/17
• Unlocking downstream processing bottleneck: Integrating cell harvest with affinity enhanced purification of monoclonal antibodies in aqueous two-phase systems using a dual tag ligand, Microbiotec15 – Congress of Microbiology and Biotechnology 2015, Évora, Portugal, 12/01/15
• Capture of monoclonal antibodies using aqueous two-phase systems, E3 Forum 2014 – Education, Employment and Entrepreneurship, Lisboa, Portugal, 05/01/14

• Immunodetection of lipoxidation products: Epitope mapping of 4-hydroxy-2-nonenal modified-human serum albumin using two different polyclonal antibodies, MASSTRPLAN Final Conference: Advances in the Study of Lipid and Protein Oxidation: From Methods to Targets, Ghent, Belgium, 03/01/19
• Purification Of Monoclonal Antibodies In Aqueous Two-phase Systems Using A Dual Tag Ligand, European Symposium on Biochemical Engineering Sciences, 09/01/16, Dublin, Ireland
• Development of Integrated Systems for Extraction and Purification of Monoclonal Antibodies directly from CHO Cell Cultures Supernatants using Aqueous Biphasic Systems composed of Ionic Liquids as Adjuvants, 5th PYCheM & 1st EYCheM, 5th Portuguese Young Chemists Meeting and 1st European Young Chemists Meeting, 04/26/16, Guimarães, Portugal

• Bacterial cell culturing
• Protein expression in E.coli strains and insect cells
• Protein purification (experience with aqueous two-phase systems and different types of chromatography using GE ÄKTA and BioRad NGC purifying systems)
• Protein quantification (SDS-PAGE, Bradford and BCA methods)
• DNA quantification (Picogreen and qubit assays)
• Immunoassays (ELISA, western blotting and lateral flow assays)
• Peptide synthesis and peptide arrays (experience with FMOC chemistry and SPOT-membrane peptide arrays)
• Peptide purification (experience with UltiMate 3000 HPLC and UHPLC)
• Mass Spectrometry (ESI and MALDI-TOF)
• Antigen design and preparation for immunisation (cross-linking, click and maleimide chemistries)
• Antibody kinetic characterisation using ForteBio Octet systems
• Molecular biology (PCR, gene cloning, plasmid DNA extraction & purification, and transformation)
• Phage display technology (generation and panning of Fab and scFV phage libraries)

• MASSTRPLAN Final Conference: Advances in the Study of Lipid and Protein Oxidation: From Methods to Targets, University of Ghent, 03/01/19
• FEBS Advanced Lecture Course 2018 I Redox-omic Technologies and their Application in Health and Disease, Spetses Hotel, 09/01/18
• MASSTRPLAN Network and Workshop Meeting, Centro Cardiologico Monzino, 06/01/18
• MASSTRPLAN Network & Workshop Meeting, CSIC, 03/01/18
• MASSTRPLAN Mid-term Meeting and Network Event, Aston University, 09/01/17
• MASSTRPLAN Summer School: LC-MS methods for oxPTMs in Inflammatory Disease, University of Aveiro, 07/01/17
• MASSTRPLAN Industry Training Event, AbSciex, Thermo Fisher Scientific, Waters, 04/01/17
• Midlands Innovation Flow Cytometry Meeting, University of Nottingham, 01/01/17
• MASSTRPLAN Network & Workshop Meeting, University of Leipzig, 07/01/16
• IP strategy to scientists, E3 Forum conference, 05/01/14
• IST Career Workshops: Career Development Training, Instituto Superior Técnico, Universidade Técnica de Lisboa, 04/01/14
• Workshop From good to excellent, Instituto Superior Técnico, Universidade Técnica de Lisboa, 03/01/14
• Introduction to factorial experimental design, IST, 03/01/14